De novo leaf transcriptome assembly of Bougainvillea spectabilis for the identification of genes involves in the secondary metabolite pathways.

Abstract

Bougainvillea spectabilis is known as a vital medicinal, ornamental as well as an essential oil producing plant. It is also a rich source of important secondary metabolites with several therapeutic properties. Various studies on its pharmacological and toxicological aspects have been published but there is no genomic or transcriptomic resource available in the public databases. To address this important issue, the de-novo transcriptome assembly of B. spectabilis leaf tissue has been done for the identification of genes involved in various important secondary metabolites, Single nucleotide polymorphism (SNPs) and Simple sequence repeats (SSRs). The transcriptome sequencing of B. spectabilis leaf tissue generated 79,811,024 raw reads with GC value 42.77%. The transcriptomic assembly was performed by Trinity software which generated 100,374 transcripts and 99,793 unigenes with minimum and maximum length of 201bp and 13,237bp and N50 value of 1470 and 1472 respectively. Annotation of these unigenes was performed using seven databases including NR, PFAM, GO and KEGG. Approximately, 44,302 unigenes were annotated in GO database. The KEGG pathway analysis revealed 23,102 unigenes in which 19,054 genes were assigned to five groups in KEGG and 130 biochemical pathways. The highest group among the five groups was Metabolism with 9230 unigenes. Moreover, about 63,226 SNPs and 30,333 SSRs in the leaf transcriptome of B. spectabilis were identified. To the best of my understanding it will be the first comprehensive transcriptome analysis of B. spectabilis from family Nyctaginaceae which will help as a reference line for further genomic and transcriptomic studies.