Abstract
BackgroundThe common littoral shrimp Palaemon serratus is an economically important decapod resource in some European communities. Aquaculture practices prevent the genetic deterioration of wild stocks caused by overfishing and at the same time enhance the production. The biotechnological manipulation of sex-related genes has the proved potential to improve the aquaculture production but the scarcity of genomic data about P. serratus hinders these applications. RNA-Seq analysis has been performed on ovary and testis samples to generate a reference gonadal transcriptome. Differential expression analyses were conducted between three ovary and three testis samples sequenced by Illumina HiSeq 4000 PE100 to reveal sex-related genes with sex-biased or sex-specific expression patterns.ResultsA total of 224.5 and 281.1 million paired-end reads were produced from ovary and testis samples, respectively. De novo assembly of ovary and testis trimmed reads yielded a transcriptome with 39,186 transcripts. The 29.57% of the transcriptome retrieved at least one annotation and 11,087 differentially expressed genes (DEGs) were detected between ovary and testis replicates. Six thousand two hundred seven genes were up-regulated in ovaries meanwhile 4880 genes were up-regulated in testes. Candidate genes to be involved in sexual development and gonadal development processes were retrieved from the transcriptome. These sex-related genes were discussed taking into account whether they were up-regulated in ovary, up-regulated in testis or not differentially expressed between gonads and in the framework of previous findings in other crustacean species.ConclusionsThis is the first transcriptome analysis of P. serratus gonads using RNA-Seq technology. Interesting findings about sex-related genes from an evolutionary perspective (such as Dmrt1) and for putative future aquaculture applications (Iag or vitellogenesis genes) are reported here. We provide a valuable dataset that will facilitate further research into the reproductive biology of this shrimp.
Highlights
The common littoral shrimp Palaemon serratus is an economically important decapod resource in some European communities
After removing ribosomal- and mitochondrial-related transcripts, the final ovary and testis non-redundant transcriptome consisted in 39,186 transcripts
Candidate Differentially expressed gene (DEG) to be involved in sexual development We aimed to reveal genes involved in sex determination, sex differentiation and gonadal development pathways
Summary
The common littoral shrimp Palaemon serratus is an economically important decapod resource in some European communities. Aquaculture practices prevent the genetic deterioration of wild stocks caused by overfishing and at the same time enhance the production. The biotechnological manipulation of sex-related genes has the proved potential to improve the aquaculture production but the scarcity of genomic data about P. serratus hinders these applications. The common littoral shrimp Palaemon serratus (Pennant, 1777) is a crustacean decapod with a geographical distribution ranging the Atlantic Ocean (from Scotland and Denmark to Mauritania, including Azores, Madeira and Canary Islands) and the entire Mediterranean Sea and the. Palaemon serratus fishing activity is crucial in some European communities, mainly around the British Isles, France and northern Spain [4]. Aquaculture practices might improve P. serratus production at once reduce the fishing pressure over the wild populations
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