De Novo Genome Assembly and Annotation of Leptosia nina Provide New Insights into the Evolutionary Dynamics of Genes Involved in Host-Plant Adaptation of Pierinae Butterflies.

Abstract

In interactions between plants and herbivorous insects, the traits enabling phytophagous insects to overcome chemical defenses of their host plants have evolved multiple times. A prominent example of such adaptive key innovations in herbivorous insects is nitrile specifier proteins (NSPs) that enabled Pierinae butterflies to colonize Brassicales host plants that have a glucosinolate-myrosinase defense system. Although the evolutionary aspects of NSP-encoding genes have been studied in some Pierinae taxa (especially among Pieris butterflies), the ancestral evolutionary state of NSPs is unclear due to the limited genomic information available for species within Pierinae. Here, we generate a high-quality genome assembly and annotation of Leptosia nina, a member of a small tribe, Leptosiaini. L. nina uses as its main host Capparaceae plants, one of the ancestral hosts within Pierinae. By using ∼90-fold coverage of Oxford Nanopore long reads and Illumina short reads for subsequent polishing and error correction, we constructed a final genome assembly that consisted of 286 contigs with a total of 225.8 Mb and an N50 of 10.7 Mb. Genome annotation with transcriptome hints predicted 16,574 genes and covered 98.3% of BUSCO genes. A typical NSP gene is composed of three tandem domains found in Pierinae butterflies; unexpectedly, we found a new NSP-like gene in Pierinae composed of only two tandem domains. This newly found NSP-like gene in L. nina provides important insights into the evolutionary dynamics of domain and gene duplication events relating to host-plant adaptation in Pierinae butterflies.