Abstract
DNA methylation is catalyzed by the DNA methyltransferase enzymes Dnmt1, Dnmt3a, and Dnmt3b. We have shown that Dnmt3a is essential for hematopoietic stem cell (HSC) differentiation. Conditional knockout of Dnmt3a (Dnmt3a-KO) resulted in HSCs that could not sustain blood generation after serial transplantation, while mutant HSCs accumulated in the bone marrow. As Dnmt3b is also highly expressed in HSCs, we reasoned it may also have a specific role in HSC function. We performed conditional deletion of Dnmt3b in HSCs, as well as Dnmt3a and Dnmt3b simultaneously, using the Mx1-cre system.
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