Abstract
Chinavia impicticornis is a neotropical stink bug of economic importance for various crops. Little is known about the development of the species, or the genetic mechanisms that may favor the establishment of populations in cultivated plants. Here, we conduct the first large-scale molecular study of C. impicticornis. Using tissues derived from the genitalia and the rest of the body for two immature stages of both males and females, we generated RNA-seq data, then assembled and functionally annotated a transcriptome. The de novo-assembled transcriptome contained around 400,000 contigs, with an average length of 688bp. After pruning duplicated sequences and conducting a functional annotation, the final annotated transcriptome comprised 39,478 transcripts, of which 12,665 were assigned to Gene Ontology (GO) terms. These novel datasets will be invaluable for the discovery of molecular processes related to morphogenesis and immature biology. We hope to contribute to the growing body of research on stink bug evolution and development, as well as to the development of biorational pest management solutions.
Highlights
The green stink bug Chinavia impicticornis (Hemiptera, Pentatomidae; Figure 1) is a neotropical species with wide distribution across South America
As well as C. impicticornis, many other stink bug species are responsible for hundreds of millions of dollars-worth of agricultural damage every year across the world
Genomic data are even more scarce, comprising four genomes assembled to date: the the brown marmorated stink bug (Halyomorpha halys (Stål), [14]), the brown stink bug (Euschistus heros, National Center for Biotechnology Information (NCBI) bioproject PRJNA489772), redbanded stink bug (Piezodorus guildinii, PRJNA263369), and the anchor stink bug (Stiretrus anchorago, PRJNA345234)
Summary
The green stink bug Chinavia impicticornis (Hemiptera, Pentatomidae; Figure 1) is a neotropical species with wide distribution across South America. Genomic data are even more scarce, comprising four genomes assembled to date: the the brown marmorated stink bug (Halyomorpha halys (Stål), [14]), the brown stink bug (Euschistus heros, NCBI bioproject PRJNA489772), redbanded stink bug (Piezodorus guildinii, PRJNA263369), and the anchor stink bug (Stiretrus anchorago, PRJNA345234) We target this gap by documenting and characterizing the first transcriptome for the neotropical stink bug Chinavia impicticornis (Hemiptera, Pentatomidae). We focused on the fifth instar because this is the stage where morphological sexual differentiation takes place These resources may be helpful for the growing body of evolution and development research being carried out on the green stink bug [15, 16]
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