Abstract
Tapiscia sinensis Oliv (Tapisciaceae) is an endangered species native to China famous for its androdioecious breeding system. However, there is a lack of genomic and transcriptome data on this species. In this study, the Tapiscia sinensis transcriptomes from two types of sex flower buds were sequenced. A total of 97,431,176 clean reads were assembled into 52,169 unigenes with an average length of 1116 bp. Through similarity comparison with known protein databases, 36,662 unigenes (70.27%) were annotated. A total of 10,002 (19.17%) unigenes were assigned to 124 pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Additionally, 10,371 simple sequence repeats (SSRs) were identified in 8608 unigenes, with 16,317 pairs of primers designed for applications. 150 pairs of primers were chosen for further validation, and the 68 pairs (45.5%) were able to produce clear polymorphic bands. Six polymorphic SSR markers were used to Bayesian clustering analysis of 51 T. sinensis individuals. This is the first report to provide transcriptome information and to develop large-scale SSR molecular markers for T. sinensis. This study provides a valuable resource for conservation genetics and functional genomics research on T. sinensis for future work.
Highlights
Tapiscia sinensis (Tapisciaceae) is a rare and ancient tree species native to China
This study provides a valuable resource for conservation genetics and functional genomics research on T. sinensis for future work
Of all of the unigenes, the majority (30,046 unigenes) were in the range of 201 to 1000 bp, which accounted for 57.59%. 13,723 of the unigenes (26.3%) ranged from 1001 to 2000 bp in length, while 8400 of the unigenes (16.1%) had lengths of more than 2000 bp (Figure 1)
Summary
Tapiscia sinensis (Tapisciaceae) is a rare and ancient tree species native to China. It is sporadically distributed in the Yangtze River valley and the southwestern provinces of China, as well as in the northern regions of Vietnam [1,2]. Tapiscia sinensis is a woody perennial androdioecious species with both male and hermaphrodite individuals in the population [3]. Male and hermaphroditic flowers blossom at similar times and both the male and hermaphroditic flowers produce viable pollen. The flower and fruit grow synchronously on the hermaphroditic individuals of T. sinensis [4,5]. Tapiscia was usually placed in Staphyleaceae under Sapindales, and was subsequently classified as Tapisciaceae under
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