De novo assembly and transcriptome characterization: novel insights into the temperature stress in Cryptotaenia japonica Hassk

Abstract

Cryptotaenia japonica Hassk of the Apiaceae family serves as an important vegetable and a medicinal herb in Asia and North America. High temperature leads to serious damage during summer. Here, deep transcriptome sequencing was performed to obtain information on gene expression and heat shock protein genes in C. japonica Hassk. A total of 40,734 unigenes were assembled and annotated. Gene Ontology and Clusters of Orthologous Groups were used to classify the functions of the unigenes. The pathway was also predicted based on Kyoto Encyclopedia of Genes and Genomes. The amounts of 2,791 simple sequence repeats were identified in 11,217 unigenes. To further investigate the expression under high temperature, 14 unigenes that encode CjHsp genes were selected based on the annotation of the Nr and Nt databases from C. japonica Hassk. The expression profiles of CjHsp genes under high-temperature treatments of 30 and 38 °C were analyzed using qRT-PCR in C. japonica Hassk. Results showed that these CjHsp genes were regulated under high-temperature treatment. These findings provide the first information on C. japonica Hassk transcriptome and enhance understanding on the mechanisms of gene regulation under high-temperature stress in C. japonica Hassk.