Abstract
Pigeonpea [Cajanus cajan (L.) Millsp.] is a heat and drought resilient legume crop grown mostly in Asia and Africa. Pigeonpea is affected by various biotic (diseases and insect pests) and abiotic stresses (salinity and water logging) which limit the yield potential of this crop. However, resistance to all these constraints is not readily available in the cultivated genotypes and some of the wild relatives have been found to withstand these resistances. Thus, the utilization of crop wild relatives (CWR) in pigeonpea breeding has been effective in conferring resistance, quality and breeding efficiency traits to this crop. Bud and leaf tissue of Cajanus scarabaeoides, a wild relative of pigeon pea were used for transcriptome profiling. Approximately 30 million clean reads filtered from raw reads by removal of adaptors, ambiguous reads and low-quality reads (3.02 gigabase pairs) were generated by Illumina paired-end RNA-seq technology. All of these clean reads were pooled and assembled de novo into 1,17,007 transcripts using the Trinity. Finally, a total of 98,664 unigenes were derived with mean length of 396 bp and N50 values of 1393. The assembly produced significant mapping results (73.68%) in BLASTN searches of the Glycine max CDS sequence database (Ensembl). Further, uniprot database of Viridiplantae was used for unigene annotation; 81,799 of 98,664 (82.90%) unigenes were finally annotated with gene descriptions or conserved protein domains. Further, a total of 23,475 SSRs were identified in 27,321 unigenes. This data will provide useful information for mining of functionally important genes and SSR markers for pigeonpea improvement.
Highlights
To keep pace with the growing human population and dietary requirements, food production must double in the 25 years (McCouch et al, 2013).The intensifying food demand, climate change, soil degradation, water and land shortages are putting more pressure on the productivity of the current crop-production system (Kastner et al, 2012)
Equal quantity of total RNA extracted from the bud and leaf tissue of C. scarabaeoides was used to construct cDNA library which was sequenced on Illumina MiSeq platform
The raw data has been deposited to NCBI with accession number SRA548291
Summary
To keep pace with the growing human population and dietary requirements, food production must double in the 25 years (McCouch et al, 2013).The intensifying food demand, climate change, soil degradation, water and land shortages are putting more pressure on the productivity of the current crop-production system (Kastner et al, 2012). In order to achieve gain in food availability, concentrated efforts to intensify food production and making it sustainable are required (Foley et al, 2011) Answers to meet these challenges lies in utilization of novel genetic diversity for production of crop varieties comprising attributes such as heat, Transcriptome Sequencing of Cajanus scarabaeoides (L.). The wild relatives have evolved in nature and show adaptation to extreme environmental changes (heat, cold, drought, salinity) and have the capability to withstand damage by insect pest and diseases (Dempewolf et al, 2014). Their utilization is hampered by limited availability of genomic resources
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