Abstract
Polygonatum sibiricum polysaccharides (PSPs) are used to improve immunity, alleviate dryness, promote the secretion of fluids, and quench thirst. However, the PSP biosynthetic pathway is largely unknown. Understanding the genetic background will help delineate that pathway at the molecular level so that researchers can develop better conservation strategies. After comparing the PSP contents among several different P. sibiricum germplasms, we selected two groups with the largest contrasts in contents and subjected them to HiSeq2500 transcriptome sequencing to identify the candidate genes involved in PSP biosynthesis. In all, 20 kinds of enzyme-encoding genes were related to PSP biosynthesis. The polysaccharide content was positively correlated with the expression patterns of β-fructofuranosidase (sacA), fructokinase (scrK), UDP-glucose 4-epimerase (GALE), Mannose-1-phosphate guanylyltransferase (GMPP), and UDP-glucose 6-dehydrogenase (UGDH), but negatively correlated with the expression of Hexokinase (HK). Through qRT-PCR validation and comprehensive analysis, we determined that sacA, HK, and GMPP are key genes for enzymes within the PSP metabolic pathway in P. sibiricum. Our results provide a public transcriptome dataset for this species and an outline of pathways for the production of polysaccharides in medicinal plants. They also present more information about the PSP biosynthesis pathway at the molecular level in P. sibiricum and lay the foundation for subsequent research of gene functions.
Highlights
Polygonatum sibiricum is a well-known traditional Chinese herb that has been widely applied for hundreds of years to treat many diseases in China, Korea, Japan, and other East Asian countries [1]
Polysaccharides isolated from P. sibiricum (PSPs) Content Varies among Polygonatum sibiricum Germplasms 2.1
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Summary
Polygonatum sibiricum is a well-known traditional Chinese herb that has been widely applied for hundreds of years to treat many diseases in China, Korea, Japan, and other East Asian countries [1]. In the Chinese Pharmacopoeia, “P. sibiricum” is prescribed as the dried rhizome of Polygonatum sibiricum Red., Polygonatum cyrtonema Hua, and Polygonatum kingianum Coll. Polysaccharides isolated from P. sibiricum (PSPs) are major contributors to sweet taste and have many important biological functions [3]. The results of animal and cell experiments have shown that these. Polysaccharides isolated from P. sibiricum (PSPs) are major contributors to sweet taste aInnt.dJ.hMaovl.eSmci. The results of animal and cell experiments 2hoafv1e7 shown that these compounds can promote the proliferation and enhance the viability of mesenchymal stem cells in bones [4], help prevent Alzheimer’s disease [5] and osteoporosis [6], serve as hcoympopliopuidnedms cicasn panrodmaontetitahtheeproroslcilfeerroattiicosn [a7n]d, eannhdanimceptrhoevveiaibmilmityunoof lmogeiscenfcuhnycmtioanl sste[m8].ceTllhseiinr pboolnyessac[c4h],ahriedlpe psrteruvcetnutrAeslzhaneidmecro’ms dpiosseiatisoen[5a]raendcloossteeloyporerolastiesd[6]t,osetrhveeirasbhioylpooglicipaildefumnicctsioannsd. Our results provide insight into the biosynthesis of polysaccharides in this species
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