Abstract

The physicochemical conditions favoring the action of chlorophyllase in olive fruit itself have been investigated. Different temperatures and aqueous incubation media that contained acetone were tested. The highest levels of chlorophyllase activation in whole fruits (without extraction of the enzyme) were achieved by heating the fruits at 50 o C in a buffer-acetone mixture. A study of the effect of pH on chlorophyllase activation in the fruit itself showed a maximum of activity at pH 7.5

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