DDX10 Promotes the Proliferation and Metastasis of CRC Cells via RPL35

Abstract

Colorectal cancer (CRC) has become the second deadliest cancer worldwide and severely threatens human health. More and more studies focus on the role of The RNA helicase DEAD-box (DDX) family in CRC. However, mechanism of DDX10 in CRC has not been elucidated. In the present study, we identified DDX10 as being highly expressed in CRC by TCGA and GEO databases. DDX10 expression was measured by immunochemical staining in 86 CRC patients. Biological roles of DDX10 were explored by cell proliferation, migration and invasion in vitro and in vivo, cell cycle assays, and using DDX56‐knockdown or overexpressed CRC cells. We found that DDX10 decreasing markedly reduced CRC cell proliferation, migration and invasion. We constructed the protein-protein interaction network (PPI) by STRING. Using LC-MS/MS and Co-IP experiments, we discovered that RPL35 is the downstream protein of DDX10. In addition, we conjecture RPL35 is related to E2F1 and immune response in CRC. In conclusion, our study helps us understand the molecular mechanisms of DDX10 in CRC, and provide candidate targets for diagnosis and treatment of CRC. Funding Information: This study was supported by the project of Suzhou Technology Bureau (No. SYS2018055), Suzhou health talent project (No. GSWS2020037), medical talent project of Jiangsu Commission of Health (No. QNRC2016873), the project of research and practice innovation plan for Postgraduates in Jiangsu Province (No. KYCX20-2721). Declaration of Interests: The authors declare that they have no conflicts of interest. Ethics Approval Statement: The study was approved by the ethics committee of the Second Affiliated Hospital of Soochow University. All patients signed the informed consent form.