Abstract
The incorporation of [ 14 C]phenylalanyl-tRNA into peptide was measured in cell-free preparations obtained from the liver of control rats and the liver of rats that had received DDT. Elongation factors 1 (EF 1) and 2 (EF 2), necessary for the binding of aminoacyl-tRNA to ribosomes and for the translocation of peptidyl-tRNA from the A site to the P site of ribosomes, were present in the pH 5 supernatant fractions of liver of control or DDT-treated rats and these fractions were incubated with saltwashed ribosomes obtained from the liver of control rats. The results provided evidence that the increased incorporation observed with the pH 5 supernatant fraction obtained from the DDT-treated rats could not be attributed to decreased ribonuclease activity or to increased EF 2 activity but was due to an increase in EF 1 activity. When EF 1 was separated from EF 2 by gel filtration chromatography, the activity of EF 1 from DDT-treated rats was greater than the activity of EF 1 from the control rats. Moreover, an increased binding of [ 14 C]phenylalanyl-tRNA to ribosomes was found in the preparations obtained four the liver of DDT-treated rats, in further support of the conclusion that aminoacyl-tRNA binding is increased in rat liver after DDT treatment.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
