Abstract
The design of cargo carriers with high biocompatibility, unique morphological characteristics, and capability of strong bonding of fluorescent dye is highly important for the development of a platform for smart imaging and diagnostics. In this paper, BODIPY-doped silica nanoparticles were prepared through a “one-pot” soft-template method using a sol-gel process. Several sol-gel precursors have been used in sol-gel synthesis in the presence of soft-template to obtain the silica-based materials with the most appropriate morphological features for the immobilization of BODIPY molecules. Obtained silica particles have been shown to be non-cytotoxic and can be effectively internalized into the cervical cancer cell line (HeLa). The described method of synthesis allows us to obtain silica-based carriers with an immobilized fluorescent dye that provide the possibility for real-time imaging and detection of these carriers.
Highlights
The chemotherapeutic delivery systems, such as polymer conjugates [1,2], micelles [3], liposomes [4,5], dendrimers [6], and organic/inorganic hybrid nanoparticles [7], have been widely explored as diagnostic devices in cancer research
The uptake ratios of amino-SiNPs-1@Boron dipyrrins (BODIPYs)-2 increased from 7.81% to 88.76% after 10 min to 24 h incubation. These results demonstrate that amino-SiNPs-1@BODIPY-2 is readily internalized by tumorous cells
Was determined that thewith morphology final silica-based materials was depended on the type of used silica-based materials definiteofmorphology, size, structure, and functional groups
Summary
The chemotherapeutic delivery systems, such as polymer conjugates [1,2], micelles [3], liposomes [4,5], dendrimers [6], and organic/inorganic hybrid nanoparticles [7], have been widely explored as diagnostic devices in cancer research. In the case of fluorescence-based sensing and bio-imaging applications for living systems, it is highly desirable to develop fluorophores with high quantum yield and light stability [8]. Many fluorescent dyes are currently using for biomedical imaging analysis [9], such as cyanine dyes [10], tetrapyrrole dyes [11], thiazide/oxazine dyes [12] They have several disadvantages, associated with poor light stability, small Stokes shift, and poor water solubility. The variety of chemical structure of BODIPY molecules makes them promising for functionalization of different drug carriers In this case, the functionalization of drug carriers using BODIPY dyes provides an excellent opportunity to develop therapeutic nanoplatforms that possess high fluorescence quantum yields for in vitro and in vivo imaging. BODIPY dyes in this case are good model compounds allowing to demonstrate the features and risks of dye immobilization and allowing to move further to the new functional dye classes and groups
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