Abstract
BackgroundBacterial pathogenicity represents a major public health concern worldwide. Secretion systems are a key component of bacterial pathogenicity, as they provide the means for bacterial proteins to penetrate host-cell membranes and insert themselves directly into the host cells’ cytosol. Burkholderia mallei is a Gram-negative bacterium that uses multiple secretion systems during its host infection life cycle. To date, the identities of secretion system proteins for B. mallei are not well known, and their pathogenic mechanisms of action and host factors are largely uncharacterized.DescriptionWe present the Database of Burkholderia malleiSecretion Systems (DBSecSys), a compilation of manually curated and computationally predicted bacterial secretion system proteins and their host factors. Currently, DBSecSys contains comprehensive experimentally and computationally derived information about B. mallei strain ATCC 23344. The database includes 143 B. mallei proteins associated with five secretion systems, their 1,635 human and murine interacting targets, and the corresponding 2,400 host-B. mallei interactions. The database also includes information about 10 pathogenic mechanisms of action for B. mallei secretion system proteins inferred from the available literature. Additionally, DBSecSys provides details about 42 virulence attenuation experiments for 27 B. mallei secretion system proteins. Users interact with DBSecSys through a Web interface that allows for data browsing, querying, visualizing, and downloading.ConclusionsDBSecSys provides a comprehensive, systematically organized resource of experimental and computational data associated with B. mallei secretion systems. It provides the unique ability to study secretion systems not only through characterization of their corresponding pathogen proteins, but also through characterization of their host-interacting partners.The database is available at https://applications.bhsai.org/dbsecsys.
Highlights
Bacterial pathogenicity represents a major public health concern worldwide
DBSecSys provides a comprehensive, systematically organized resource of experimental and computational data associated with B. mallei secretion systems
Host-pathogen Protein-protein interaction (PPI) are available for download in the Proteomics Standards Initiative Molecular Interactions (PSI-MI) Tab 2.5 format [45]
Summary
We developed a curated database of bacterial secretion system proteins and their host factors. The database contains comprehensive information about B. mallei proteins associated with secretion systems, their involvement in virulence, their host targets (proteins), and their mechanisms of action inferred from literature and host-pathogen interaction data. Systematical organization of experimental and computational data allows for efficient data retrieval, browsing, querying, and visualization, and enables users to study secretion systems through characterization of the corresponding pathogen proteins and through characterization of host-interacting partners. Together, these features make DBSecSys a unique resource for B. mallei secretion system research. All authors read and approved the final manuscript
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