Daytime of leaf wounding determines plant biomass and affect the interplay between growth and defense in Brassica crops.

Abstract

Recent discoveries pointed out the importance of the mutual correlation between timing of environmental stress and plant fitness. However, the internal reshaping of plant growth under daily stress sensing, and their metabolic coordination remain to be investigated. Thus, we aim to study the connection between daytime, growth and defense to understand how plant fitness is affected by diurnal stress inputs. To accomplish that, we examined if simulated herbivory (leaf wounding) in the morning, mid-day or evening differentially influenced plant defensive states vs growth in three different crop species of the Brassica genus, broccoli (Brassica oleraceae), turnip greens (B. rapa) and rapeseed (B. napus). The data presented here revealed that plant's ability to tolerate wounding stress is diurnally regulated in Brassica crops. Trade-offs between plant biomass and investment in glucosinolates (GSL) and phenolics were affected by daytime of leaf stress. Negative correlations between plant biomass and induction of defensive compounds were found for morning and evening treated plants. However, these correlations were positives for mid-day treatment. Interestingly, we revealed new connection between plant growth and changes of aliphatic GSLs and flavonoids in response to wounding. These data suggest that metabolic stress-dependent circadian oscillations of leaves defenses could be one mechanism conferring competitive advantage to plants able to anticipate daily environmental variations by synchronize growth with them. Moreover, this work provides first insights of how secondary metabolites are linked to growth response in a timely manner.