Date Seeds Supplementation Reduces Stress Oxidative, Inflammation and Prevents Anxiety-like Behavior in Nicotinamide-Streptozotocin-induced Diabetic Rat Model

Abstract

Background: several researches indicate that type 2 diabetes is associated with brain biochemical and behavioral perturbations. Objective: this study aims to explore the neuroprotective effect of date seeds phenolic compounds on hyperglycemia induced inflammation, oxidative stress and anxiety-like behavior in the brain of diabetic rats. Method: Rats were assigned to 4 groups (of 10 rats each) as follows: Group 1 (normal control), group 2 was diabetic control, while group 3 and group 4 were diabetic treated with date seeds extract (DSE) and metformin respectively. Experimental type 2 diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ) (65 mg/kg) 15 min after nicotinamide (NA) intraperitoneal injection (110 mg/kg). After diabetes confirmation, Treatment lasted for 6 weeks. Before sacrifice, anxiety-like behavior of all rats was assessed using 4 behavioral tests. After sacrifice, lipid peroxidation and tumor necrosis factor-α levels were evaluated in the brain (Hippocampus and prefrontal cortex) of all groups rat. Result: treatment with date seeds extract (2000 mg/kg) and/or metformin (300mg/kg) significantly decreased the level of lipid peroxidation levels and control the activity of tumor necrosis factor-α in Hippocampus as well as in prefrontal cortex in NA-STZ diabetic rats. Furthermore, the administration of date seeds extract or Metformin for 6weeks prevented anxiety-like behavior in these rats. Conclusion: in conclusion, the findings of the current study demonstrated that date seeds exhibited anxiolytic effect in type 2 diabetes, that could be due to its strong antioxidant (suppress oxidative stress) and anti-inflammatory (reduce pro-inflammatory cytokines) activities.