Abstract
Datasets from a slow carbonate vapor diffusion and mineral precipitation protocol for Dictyostelium ECM and cellulose stalks show examples for composite materials obtained by an in vitro approach, which differs substantially from the in vivo approach reported in The Journal of Structural Biology, doi: 10.1016/j.jsb.2016.03.015 [1]. Methods for obtaining the datasets include bright field transmitted light microscopy, fluorescence microscopy, LC-PolScope birefringence microscopy, variable pressure scanning electron microscopy (VP-SEM/ESEM), and Raman imaging spectroscopy.
Highlights
Datasets from a vapor diffusion mineral precipitation protocol for Dictyostelium stalks Magdalena Eder, Christina Muth, Ingrid M
Data could serve for comparison with Dictyostelium extracellular matrix (ECM), where minerals were precipitated using alternative methods
Datasets show fluorescence light microscopy, LC-PolScope/Abrio imaging, scanning electron microscopy (VP-SEM), and confocal Raman imaging spectrometry data of composite materials obtained from Dictyostelium extracellular matrix (ECM)
Summary
Raw, analyzed (overlay images) Native biological samples (Dictyostelium ECM) were subjected to slow precipitation of calcium carbonate in aqueous Ca2 þ solution with ammonium carbonate in the vapor phase Specimens were investigated in wet or dry conditions, without any further modification n/a. Data could serve for comparison with minerals precipitated without organic matrix. Data could serve for comparison with calcifying ECM of other cell types. Data could serve for comparison with Dictyostelium ECM, where minerals were precipitated using alternative methods. Datasets show fluorescence light microscopy, LC-PolScope/Abrio imaging, scanning electron microscopy (VP-SEM), and confocal Raman imaging spectrometry data of composite materials obtained from Dictyostelium extracellular matrix (ECM). Different parts of native and genetically modified ECM were subjected to slow precipitation of calcium carbonate in aqueous Ca2 þ solution with ammonium carbonate in the vapor phase. Datasets of specimens modified with different protein derivatives, either from egg shell (OC-17) or from mollusc shells (n16N, perlucin) investigated in wet or dry conditions and without any further modification are presented
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