Abstract
We used Nile red to estimate lipid content in oleaginous yeasts using a high-throughput approach. We measured the fluorescence intensity of Nile red using different solvents, yeast strains, and incubation times in optimized excitation/emission wavelengths. The data show the relative fluorescence units (RFU) for Nile red excitation, using 1× PBS, 1× PBS and 5% v/v isopropyl alcohol, 50% v/v glycerol, culture medium A-gly broth, and A-gly broth supplemented with 5% v/v DMSO. In addition, we showed the RFU for the Nile red dye for different oleaginous and non-oleaginous yeast strains, such as Meyerozyma guilliermondii BI281A, Yarrowia lipolytica QU21 and Saccharomyces cerevisiae MRC164. Other measurements of lipid accumulation kinetics were shown for the above and additional yeast strains. These datasets provide the guidelines to obtain the optimal solvent system and the minimal interaction time for the Nile red dye to enter in the cells and obtain a stable readout.
Highlights
A rapid and inexpensive method for the selection of oleaginous strains out of a large collection of yeasts was based on Nile red fluorescence and a microplate reader equipment
The dataset included in the present work shows the fluorescence readings of Nile red dye using different yeast species, solvents and incubation times
The first part of the dataset reports the fluorescence reads for each variable against the incubation time
Summary
Nile red (9-diethylamino-5H-benzo[α]phenoxazine-5-one) is one of the most commonly used dyes to quantify neutral lipids in yeasts [1,2,3,4,5,6]. A mixture of DMSO with culture media A (including the cells) was mixed with the Nile red dye, measuring the fluorescence kinetics for 20 min with 60-s intervals to detect the fluorescence peak [2]. The second part shows the fluorescence readings at different incubation times, including several lipid-accumulation kinetics of different oleaginous yeasts This dataset could be used to predict oleaginous microorganisms using mathematical modeling, to define if the oleaginous ability is species-specific or strain-specific. It could help to determine the right incubation time or solvent for each strain, to compare the oleaginous ability of the strains used in this study with other strains
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