Dataset of milk whey proteins of three indigenous Greek sheep breeds

Athanasios K Anagnostopoulos,Angeliki I Katsafadou,Vasileios Pierros,Evangelos Kontopodis,George C Fthenakis,George Arsenos,Spyridon Ch Karkabounas,Athina Tzora,Ioannis Skoufos,George Th Tsangaris

doi:10.1016/j.dib.2016.06.040

Abstract

The importance and unique biological traits, as well as the growing financial value, of milk from small Greek ruminants is continuously attracting interest from both the scientific community and industry. In this regard the construction of a reference dataset of the milk of the Greek sheep breeds is of great interest. In order to obtain such a dataset we employed cutting-edge proteomics methodologies to investigate and characterize, the proteome of milk from the three indigenous Greek sheep breeds Mpoutsko, Karagouniko and Chios. In total, more than 1300 protein groups were identified in milk whey from these breeds, reporting for the first time the most detailed proteome dataset of this precious biological material. The present results are further discussed in the research paper “Milk of Greek sheep and goat breeds; characterization by means of proteomics” (Anagnostopoulos et al. 2016) [1].

Highlights

Milk samples of the Mpoutsko, Karagouniko and Chios indigenous Greek sheep breeds, were collected by milking at three different time points, throughout the lactation period, on various farms and flocks in regions scattered across Greece (Fig. 1)
200 ng whey fractions were treated with urea and triethyl ammonium bicarbonate (TEAB) under mild sonication in a water-bath for 30 min and reduction and alkylation steps of proteins were carried out using dithiothreitol and iodoacetamide solutions, The final step of processing included overnight digestion of extracted proteins of all samples by trypsin (Roche Diagnostics, Basel, Swiss) at a final concentration of 500 ng/μl
Full-scan data were acquired at a resolving power of 60,000 with a maximum integration time of 250 ms

Summary

Data accessibility

Veterinary science Mpoutsko, Karagouniko and Chios sheep milk proteome. Analyzed Milk samples from the indigenous Greek sheep breeds Mpoutsiko, Karagouniko and Chios, were systematically collected and analyzed in order to characterize the protein content of the milk of each breed. To report for the first time the proteome dataset of the milk whey from the three indigenous Greek sheep breeds. The comparative analysis of the data could lead to new dairy products with specific nutritional characteristics for the human health. Milk samples of the Mpoutsko, Karagouniko and Chios indigenous Greek sheep breeds, were collected by milking at three different time points, throughout the lactation period, on various farms and flocks in regions scattered across Greece (Fig. 1). The milk proteome lists comprised of 550 protein groups for the Karagouniko breed, 583 for the Mpoutsko breed and 685 protein groups for the Chios breed

Animals and sample collection

Findings

Sample preparation