Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms.

Makiko Shimizu,Hiroshi Yamazaki,Tomoki Koyama,Izumi Kishimoto

doi:10.1016/j.dib.2015.10.019

Makiko Shimizu, Hiroshi Yamazaki + Show 2 more

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https://doi.org/10.1016/j.dib.2015.10.019

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Journal: Data in Brief	Publication Date: Oct 25, 2015
Citations: 6	License type: cc-by

Affiliation: Showa Pharmaceutical University

Abstract

This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes” (Shimizu et al., Clinica Chimica Acta 441, 71–74, 2015), which presents a multiplex real-time polymerase chain reaction method with dual-labeled probes for human P450 2A6 wild-type and whole-gene deletion. Real-time methods have dramatically improved the speed of complex genetic diagnostics compared to conventional assays based on restriction enzyme digestion. Here, we show the basic assay validation data by single and multiplex determinations in comparison with commercial TaqMan copy number assays for P450 2A6.

Highlights

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wildtype cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes” (Shimizu et al, Clinica Chimica Acta 441, 71–74, 2015), which presents a multiplex realtime polymerase chain reaction method with dual-labeled probes for human P450 2A6 wild-type and whole-gene deletion
We show the basic assay validation data by single and multiplex determinations in comparison with commercial TaqMan copy number assays for P450 2A6

Summary

Data Article

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms. Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan article info. Article history: Received 22 September 2015 Received in revised form October 2015 Accepted October 2015 Available online 25 October 2015 Keywords: CYP2A6*1 CYP2A6*4 Copy number assays Real-time PCR TaqMan Whole-gene deletion

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