Database of the polymorphic genetic markers D19S433 and D2S1338 from the population of Buenos Aires Province, Argentina

Abstract

Short tandem repeat (STR) loci are the most informative DNA genetic markers for attempting to individualize biological material for application at paternity and forensic cases. Before a new marker system can be introduced into forensic casework, a database for the relevant population must be established for statistical evaluation of the evidence. Therefore, this report presents allele frequency data and parameters of biological or legal interest, such as heterozygosity value and power of exclusion in Buenos Aires Province (Argentina) population sample for the loci D19S433 and D2S1338. Blood samples ( N = 473) were collected form individuals unrelated throughout Buenos Aires Province on FTA cards. Following DNA extractions, multiplex PCR amplifications were carried out using AmpF lSTR Identifiler PCR Amplification kit (Applied Biosystems) and amplified products analyzed on an Applied Biosystems DNA sequencer (Model 3130). Heterozygosity value is estimated as 0.81184 for D19S433, and 0.85201 for D2S1338. Both STR loci are in Hardy–Weinberg equilibrium. For D19S433, power of exclusion and power of discrimination are estimated as 0.621 and 0.937, respectively. For D2S1338 the same parameters are 0.699 and 0.968. The allele frequency data can be used for deriving estimates of multiple locus profile frequencies for identity testing purposes.