Data supporting the role of Fyn in initiating myelination in the peripheral nervous system.

Yuki Miyamoto,Junji Yamauchi,Shuji Takada,Kazuaki Nakamura,Kazuko Kawahara,Moe Tamano,Tomohiro Torii,Akito Tanoue

doi:10.1016/j.dib.2016.03.096

Abstract

Transgenic mice, which express active Fyn tyrosine kinase under the control of a glial fibrillary acidic protein promoter, have been produced. This promoter induces protein expression in the initiation stage of myelination in the peripheral nervous system (PNS) “Phosphorylation of cytohesin-1 by Fyn is required for initiation of myelination and the extent of myelination during development (Yamauchi et al., 2015 [1])”. Herein we provide the data regarding myelination-related protein markers and myelin ultrastructure in transgenic mice.

Highlights

Transgenic mice, which express active Fyn tyrosine kinase under the control of a glial fibrillary acidic protein promoter, have been produced. This promoter induces protein expression in the initiation stage of myelination in the peripheral nervous system (PNS) “Phosphorylation of cytohesin-1 by Fyn is required for initiation of myelination and the extent of myelination during development (Yamauchi et al, 2015 [1])”
This data set is of value to the scientific community to need the information for molecules controlling myelination
A DNA fragment ( 4.5 kb) containing the SV40 enhancer, a mouse glial fibrillary acidic protein (GFAP) promoter specific for the neonatal stage of Schwann cells in the PNS [1,6,7], V5-epitopetagged active Fyn, an artificial intron, and human chorionic gonadotropin polyA units [1,7] was digested from the vector backbone ( 3.5 kb) with NcoI, purified, and injected into fertilized BDF1 oocytes

Summary

Data from Fyn transgenic mice

Neonatal transgenic mice expressing active Fyn exhibited increased expression levels of myelin marker proteins such as myelin protein zero (MPZ, called P0) and 20,30-cyclicnucleotide 30-phosphodiesterase (CNPase) (Fig. 1A and B). Transgenic mice exhibited smaller g-ratios, indicating increased myelin thickness, in the sciatic nerves (0.73 70.045 in the transgenic mice compared to 0.78 70.060 in the control mice). The myelination-associated transcription factor Krox20 [4,5] was increased in transgenic mouse nerves (Fig. 4, A and B). Levels of Sox (Fig. 5A and B) and Oct (Fig. 6A and B), transcription factors expressed in Schwann cell lineage cells [4,5], were comparable in transgenic mice and controls

Generation of active Fyn transgenic mice

Immunoblotting

Electron microscopic analysis