Data Supplement from Neuronal Pentraxin 2 Supports Clear Cell Renal Cell Carcinoma by Activating the AMPA-Selective Glutamate Receptor-4

Abstract

<p>Supplementary Figure 4. Measurement of intracellular calcium influx into (A) empty vector or (B) GluR4 transfected Caki1 cells treated with 1ng/µL R-NPTX2 and DMSO or CFM-2 (10µM) over time using the cell permeable fluorescently labeled calcium indicator Calcium GreenTM-1, AM. Results are calculated as change in fluorescence over time (min). (*) Denotes significant change in fluorescence as compared to DMSO control per time point, and (**) denotes a significant change between R-NPTX2 +/- CFM-2 treated cells. (C) Intracellular Ca2+ levels of NPTX2 and NPTX2-GluR4 transfected Caki1 cells pretreated for 1 hour with DMSO or specified doses of CFM-2. (*) Denotes a significant change in fluorescence of NPTX2 and NPTX2-GluR4 as compared to EV control, and (**) indicates significant changes in fluorescence between DMSO and CFM-2 treated groups</p>