Abstract
The Suppressor of Clear, Caenorhabditis elegans Homolog (SHOC2) is a scaffold protein that positively modulates activity of the RAS/ERK1/2 MAP kinase signaling cascade. We set out to understand the ERK1/2 pathway transcriptional response transduced through the SHOC2 scaffolding module. This data article describes raw gene expression within triplicates of kidney fibroblast-like Cos1 cell line expressing non-targeting shRNA (Cos-NT) and triplicates of Cos1 cells depleted of SHOC2 using shRNA (Cos-LV1) upon activation of ERK1/2 pathway by the Epidermal Growth Factor Receptor (EGFR). The data referred here is available in NCBI׳s Gene Expression Omnibus (GEO), accession GEO: GSE67063 as well as NCBI׳s Sequence Read Archive (SRA), accession SRA: SRP056324. A complete analysis of the results can be found in “Shoc2-tranduced ERK1/2 motility signals – Novel insights from functional genomics”(Jeoung et al., 2016) [1].
Highlights
The Suppressor of Clear, Caenorhabditis elegans Homolog (SHOC2) is a scaffold protein that positively modulates activity of the RAS/ ERK1/2 MAP kinase signaling cascade
While the activation of RAF, MEK, and ERK kinases in the ERK1/2 signaling pathway have been studied extensively, little is known about the activity of the ERK1/2 pathway in context of specific scaffolding modules
This dataset provides a novel look into the transcriptional response mediated through the SHOC2/ERK1/2 signaling axis, which can give greater insight into the mechanisms regulating signals of the ERK1/2 pathway [1]
Summary
Transcriptome, table, figure High-throughput RNA sequencing using Illumina HiSeq 2500. While the activation of RAF, MEK, and ERK kinases in the ERK1/2 signaling pathway have been studied extensively, little is known about the activity of the ERK1/2 pathway in context of specific scaffolding modules This dataset provides a novel look into the transcriptional response mediated through the SHOC2/ERK1/2 signaling axis, which can give greater insight into the mechanisms regulating signals of the ERK1/2 pathway [1]. This dataset becomes only the third high throughput sequencing transcriptional profile for Cos, yielding to the potential for generalized transcriptome studies of the Cos cell line This data consists of six high-throughput sequencing samples of Shoc depleted (n 1⁄4 3) or not depleted (n1⁄4 3) Cos cells generated from an Illumina HiSeq 2000.
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