Abstract
This data article describes cellular dynamics, such as migration speed and mobility of the cytoskeletal protein, of wild-type human fibroblast cells and cells with a modified adeno-associated virus integration site 1 (AAVS1) locus on human chromosome 19. Insertion of exogenous gene into the AAVS1 locus has been conducted in recent biological researches. Previously, our data showed that the AAVS1-modification changes cellular contractile force (Mizutani et al., 2015 [1]). To assess if this AAVS1-modification affects cell migration, we compared cellular migration speed and turnover of cytoskeletal protein in human fibroblasts and fibroblasts with a green fluorescent protein gene knocked-in at the AAVS1 locus in this data article. Cell nuclei were stained and changes in their position attributable to cell migration were analyzed. Fluorescence recovery was observed after photobleaching for the fluorescent protein-tagged myosin regulatory light chain. Data here are related to the research article “Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 85” [1].
Highlights
Our data showed that the associated virus integration site 1 (AAVS1)-modification changes cellular contractile force (Mizutani et al, 2015 [1])
Fluorescence recovery was observed after photobleaching for the fluorescent protein-tagged myosin regulatory light chain
Data here are related to the research article “Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 85” [1]
Summary
Image, graph, figure Phase contrast and wide-field fluorescent (cell nucleus tracking) images were acquired using an inverted microscope (TE2000; NIKON, Tokyo, Japan) equipped with a digital CMOS camera (ORCA-Flash2.8; Hamamatsu Photonics K.K., Shizuoka, Japan). Cell tracking data from wild-type and AAVS1-modified cells are available for computer simulation of cell migration. Data from fluorescence recovery after photo bleaching for the fluorescent protein-tagged myosin regulatory light chain are applicable for analysis of myosin binding and diffusion coefficient in wild-type and AAVS1-modified cells. These data may be used as a benchmark of evaluation of the side effect of AAVS1-modified cells. The data describes the cell migration speed and the dynamical behavior of myosin regulatory light chain (MRLC) of wild-type human fibroblasts (WT cells) and AAVS1-modified cells
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