Abstract
The use of new compounds as dietary supplements is increasing, but little is known in terms of possible consequences of their use. Pluripotent stem cells are a promising research tool for citotoxicological research for evaluation of proliferation, cell death, pluripotency and differentiation. Using the mouse embryonic stem cell (mESC) model, we present data on three different compounds that have been proposed as new potential supplements for co-adjuvant disease treatments: kaempferol, berberine and Tauroursodeoxycholic acid (TUDCA). Cell number and viability were monitored following treatment with increased concentrations of each drug in pluripotent culture conditions.
Highlights
Data on the potential impact of food supplements on the growth of mouse embryonic stem cells
The use of new compounds as dietary supplements is increasing, but little is known in terms of possible consequences of their use
Using the mouse embryonic stem cell model, we present data on three different compounds that have been proposed as new potential supplements for co-adjuvant disease treatments: kaempferol, berberine and Tauroursodeoxycholic acid (TUDCA)
Summary
Analyzed Kaempferol, berberine and TUDCA were added to culture mouse embryonic stem cells (mESC) with different concentrations for three days; pictures were acquired and colony size as cell numbers assessed. 200 mM kaempferol induce a significant reduction of mouse embryonic stem cell number. Analyzed Kaempferol, berberine and TUDCA were added to culture mouse embryonic stem cells (mESC) with different concentrations for three days; pictures were acquired and colony size as cell numbers assessed. Tauroursodeoxycholic acid – TUDCA [50–250 mM] did not affect embryonic stem cell growth after 3 days in culture. Taking advantage of embryonic stem cells as a citotoxicological model [1,2,3,4], we monitored cell number and viability after acute exposure of mESCs to kaempferol [1], berberine and Tauroursodeoxycholic acid (TUDCA), potential supplements for co-adjuvant disease treatments. On every daily media supplementation, a new aliquot was used
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