Data on the effect of in vivo knockdown using artificial ErbB3 miRNA on Remak bundle structure

Yuki Miyamoto,Tomohiro Torii,Kazuko Kawahara,Masashi Inoue,Takako Morimoto,Masahiro Yamamoto,Junji Yamauchi

doi:10.1016/j.dib.2017.04.014

Yuki Miyamoto, Tomohiro Torii + Show 5 more

Open Access

https://doi.org/10.1016/j.dib.2017.04.014

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Abstract

Mature Schwann cells, the peripheral nervous system (PNS) glial cells, have two major roles for neuronal axons (Bunge, 1993) [1]. For large diameter axons, Schwann cells form myelin sheaths with multiple layers. For small diameter axons, they form Remak bundle composed only of single layer of the Schwann cell plasma membrane. In the PNS, ErbB3 forms a dimer with ErbB2 on the Schwann cell plasma membrane. ErbB3 plays a key role in myelination by myelinating Schwann cells, that is to say, its role in myelin thickness. Herein we provide the data regarding the effect of in vivo knockdown of ErbB3 on the thickness between an axon and a neighboring axon in Remak bundle, which is formed by non-myelinating Schwann cells. Since ErbB3 knockout mice are embryonically lethal, Schwann cell lineage-specific transgenic mice transcribing ErbB3 shRNA with an artificial miRNA backbone were generated and used in these experiments (Torii et al., 2014) [2].

Highlights

Data on the effect of in vivo knockdown using artificial ErbB3 miRNA on Remak bundle structure
In the peripheral nervous system (PNS), ErbB3 forms a dimer with ErbB2 on the Schwann cell plasma membrane
ErbB3 plays a key role in myelination by myelinating Schwann cells, that is to say, its role in myelin thickness

Summary

Data from ErbB3 shRNA transgenic mice

While myelin is composed of multiple layer plasma membranes of myelinating Schwann cells, Remak bundle is axonal one with single layer of the plasma membranes of non-myelinating Schwann cells [1]. In Schwann cell lineage-specific ErbB3 shRNA transgenic mice [2]) and littermate controls, axon diameter 2 and 3; Fig. 5) in Remak bundle were comparable. In ErbB3 shRNA transgenic mice, the distance between an axon and a. 2 and 3; Fig. 6), showing the role of Schwann cell ErbB3 in Remak bundle formation Neighboring axon was significantly short (Figs. 2 and 3; Fig. 6), showing the role of Schwann cell ErbB3 in Remak bundle formation

ErbB3 shRNA transgenic mice

Electron microscopy

Immunoblotting

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