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Abstract
Obesity and metabolic diseases are closely associated with insulin resistance. Obesity-induced miRNAs are also considered to be potential contributors to the development of insulin resistance and type 2 diabetes. Previously, the expression of miR-1271 was reported to be upregulated in the liver of diet-induced obese mice (Yang et al., 2016) [1]. In this data article, multiple in silico analysis predicted FOXO1 gene to be a direct target of miR-1271. Dual luciferase reporter gene analysis showed that miR-1271 suppressed FOXO1 expression by direct binding to 3′UTR. The overexpression of miR-1271 reduced the protein expression of FOXO1, thereby reducing the transcription of PEPCK, a downstream target of FOXO1. The data is related to a research article entitled "MiR-1271 upregulated by saturated fatty acid palmitate provokes impaired insulin signaling by repressing INSR and IRS-1 expression in HepG2 cells" (Yang et al., 2016) [1].
Highlights
Obesity and metabolic diseases are closely associated with insulin resistance
Multiple in silico analysis predicted FOXO1 gene to be a direct target of miR-1271
The data is related to a research article entitled "MiR1271 upregulated by saturated fatty acid palmitate provokes impaired insulin signaling by repressing INSR and IRS-1 expression in HepG2 cells" (Yang et al, 2016) [1]
Summary
Figure and text Analysis of Dual luciferase reporter gene assay and immunoblotting Analyzed Transfection of miR-1271, Analysis of FOXO1 and PEPCK expression. A dysregulation of miR-1271 expression was reported to be linked causally to the development of hepatic insulin resistance [1] This data article assessed the targets of miR-1271 on the insulin signaling pathway using in silico analysis, such as TargetScan, Pictar, and miRWalk. Luciferase reporter constructs containing either a tentative miR-1271 target sequence in FOXO1 3′UTR (wild-type; FOXO1 3Uwt), or three nucleotides mutant of the tentative target sequence (FOXO1 3Umut) were generated in the pmirGLO vector (Fig. 1B), as described in the Section 2. These reporter constructs, which included a Firefly luciferase cassette to allow normalization of the internal Renilla luciferase activity, were transfected transiently together with a scRNA control or miR-1271 mimic. Insights into the implications of miR-1271 on the pathogenesis of insulin resistance and type 2 diabetes are reported elsewhere [1]
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