Abstract
Verticinone as a steroidal alkaloid is one of the major active constituents of medicinal herb, Fritillaria imperialis with several pharmacological properties. Present data demonstrate an in vitro assessment of verticinone effects on β-TC6 pancreatic and C2C12 skeletal muscle cells include cell survival, activities of carbohydrate-hydrolyzing enzymes (α-amylase and α-glucosidase), levels of insulin secreted into the media, glucose uptake ability, advanced glycation end product (AGEs) include 3-deoxyglucosone, methylglyoxal, and pentosidine levels and the activity of glyoxalase I. Data reveals possible hypoglycemic potential of verticinone, although, the high concentrations of this compound were associated with elevated amount of AGEs and it should be assessed in future investigations.
Highlights
Data on possible in vitro anti-diabetic effects of verticinone on b-TC6 pancreatic and C2C12 skeletal muscle cells
Present data demonstrate an in vitro assessment of verticinone effects on b-TC6 pancreatic and C2C12 skeletal muscle cells include cell survival, activities of carbohydratehydrolyzing enzymes (a-amylase and a-glucosidase), levels of insulin secreted into the media, glucose uptake ability, advanced glycation end product (AGEs) include 3-deoxyglucosone, methylglyoxal, and pentosidine levels and the activity of glyoxalase I
Data reveals possible hypoglycemic potential of verticinone, the high concentrations of this compound were associated with elevated amount of AGEs and it should be assessed in future investigations
Summary
The assessment of cell viability was performed according to the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) colorimetric assay. After independent treatment of the C2C12 and b-TC6 cells with verticinone, cells were washed and incubated with MTT solution to reach the desired final concentration and after 3 h, dimethyl sulfoxide (DMSO) added. The absorbance ratio of the verticinone treated cells to the absorbance of DMSO-treated control cells was determined using spectrophotometrically at 570 nm (UV-VIS) by the Multi-Label Reader (Hidex, Turku, Finland). The cell suspensions, sodium phosphate buffer and high purity aamylase solutions were incubated at room temperature for 10 min. A starch solution in sodium phosphate buffer was added. Acarbose considered as a positive standard and the absorbance of reaction mixtures was measured at 540 nm (UV-VIS) using multimode-reader [7]. A-glucosidase from Saccharomyces cerevisiae was added to cell suspensions and the obtained mixture incubated with phosphate buffer solution for 5 min at 37 C. The reaction was stopped by the addition of Na2CO3 and the absorbance was determined at 405 nm [8]
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