Abstract
Previously, we reported that occupational exposure to formaldehyde (FA) exposure in factory workers reduced platelet counts, http://dx.doi.org/10.1158/1055-9965.EPI-09-0762[1], while exposure in mice increased platelet counts http://dx.doi.org/10.1371/journal.pone.0074974[2]. Bone marrow megakaryocyte (MK) numbers were also increased in exposed mice, as determined qualitatively. The data presented here are from a quantitative evaluation of MK numbers in the bone marrow histopathological slides from the previous FA exposure experiments in mice. Bone marrow slides were prepared using a single 5 μm section of femur from 2 mice randomly selected from each exposure group (n=9) treated with 0, 0.5 and 3.0 mg/m3 FA by nose-only inhalation. MKs were systemically counted and average MK frequency was calculated as the total MK per slide divided by the number of fields evaluated. Data are presented visually as microscopy views and graphically as MK frequency.
Highlights
Subject area More specific subject area Type of data How data was acquired Data format Experimental factors
Bone marrow histology slides were prepared from 2 mice randomly selected from each exposure group and MKs were counted
Confirms that MK numbers are increased in the bone marrow of FA-exposed mice in a dose-dependent manner
Summary
Bone marrow histology slides were prepared from 2 mice randomly selected from each exposure group and MKs were counted. Confirms that MK numbers are increased in the bone marrow of FA-exposed mice in a dose-dependent manner. Data on MKs from FA-exposed and control mice are presented qualitatively (representative microscopy fields, Fig. 1) and quantitatively (MK frequencies, Fig. 2). The resulting MK frequency (the total number of MKs divided by the total number of scorable fields) was significantly increased in the FA-exposed mice at 0.5 mg/m3 (13.67, p o0.01) and 3.0 mg/m3 (15.50, p o0.01) compared with the unexposed control group (7.27) (Fig. 2) and a dose-dependent effect was apparent (ptrend1⁄4 0.001). The MK frequency data quantitatively shows that MK numbers are increased in FA-exposed mice compared with controls (Fig. 2)
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