Abstract
Australian native plants have a long history of therapeutic use in indigenous cultures particularly for the treatment of wounds. We analysed 14 plant derived compounds from the species Pilidiostigma glabrum, Myoporum montanum, Geijera parviflora, and Rhodomyrtus psidioides for keratin 1, 5, 10 and 14 supporting the research article “Native Australian plant extracts differentially induce Collagen I and Collagen III in vitro and could be important targets for the development of new wound healing therapies” [5]. An in situ immunofluorescence assay was used in a 96 well tissue culture plate format to measure keratin expression in immortalised human keratinocytes (HaCaTs) exposed Australian native plant compounds to NMR spectra for the plant extracts are included in this article as is quantitative fluorescent intensity data of keratin 1, 5, 10 and 14 expression.
Highlights
Australian native plants have a long history of therapeutic use in indigenous cultures for the treatment of wounds
Figures Fluorescence Microscopy, Olympus IX83 fluorescence microscope, Olympus DP80 camera, cellSens Dimension software Analyzed Lyophilized plant extracts were dissolved in neat DMSO
We have developed a novel 96 well format in situ immunofluorescence assay for measuring the level of different proteins present in cells. This novel assay was used to determine the effect of Australian native plant extracts on these different keratins in keratinocytes
Summary
Figures Fluorescence Microscopy, Olympus IX83 fluorescence microscope, Olympus DP80 camera, cellSens Dimension software Analyzed Lyophilized plant extracts were dissolved in neat DMSO. We have developed a novel 96 well format in situ immunofluorescence assay for measuring the level of different proteins present in cells. This novel assay was used to determine the effect of Australian native plant extracts on these different keratins in keratinocytes. This assay could be a useful tool for the quantification of specific protein levels in large numbers of samples. Data is included that shows keratin 1, 5, 10 and 14 protein expression in the cultured human keratinocyte cell line (HaCaT)
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