Abstract
The present dataset provides methodology to isolate and purify fibrinolytic enzyme from microbe isolated from the natural source. The information provided in this data article includes (1) isolation and identification of Pseudomonas baetica SUHU25, (2) optimization of cultural conditions, (3) extraction and purification of fibrinolytic enzyme, (4) protein estimation, (5) assay of fibrinolytic activity, (6) SDS PAGE for purified enzyme protein, (7) effect of pH, temperature and metal ions on fibrinolytic activity of enzyme protein, and (8) In-vitro blood clot dissolution assay.
Highlights
The present dataset provides methodology to isolate and purify fibrinolytic enzyme from microbe isolated from the natural source
The information provided in this data article includes (1) isolation and identification of Pseudomonas baetica SUHU25, (2) optimization of cultural conditions, (3) extraction and purification of fibrinolytic enzyme, (4) protein estimation, (5) assay of fibrinolytic activity, (6) SDS PAGE for purified enzyme protein, (7) effect of pH, temperature and metal ions on fibrinolytic activity of enzyme protein, and (8) In-vitro blood clot dissolution assay
The scientific fraternity looking for novel sources of fibrinolytic enzymes can use the presented data A further study on characterization of fibrinolytic enzyme and testing efficacy of enzyme by other available In-vitro and
Summary
Value of the data There is extensive research in search of a effective fibrinolytic enzymes as presently available fibrinolytic enzymes are prone to low specificity. The presented data is first report of fibrinolytic enzyme from Pseudomonas baetica SUHU25. The scientific fraternity looking for novel sources of fibrinolytic enzymes can use the presented data A further study on characterization of fibrinolytic enzyme and testing efficacy of enzyme by other available In-vitro and. In-vivo methods can be planned shows relative activity (%) of fibrinolytic enzyme of Pseudomonas baetica SUHU25 with change in carbon source, nitrogen source, pH, temperature and incubation period respectively. Fig. 11shows In-vitro blood clot dissolution by the purified enzyme preparation. 12e14 shows relative activity (%) of fibrinolytic enzyme with change in pH, temperature and presence of various metals respectively.
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