Abstract
Elettaria cardamomum (L.) Maton, or small cardamom referred as ‘queen of spices’, is a perennial herbaceous rhizomatous monocot of the family Zingiberaceae. Cardamom seeds and fruits are the economically significant parts and effectively used as a traditional medicine, food additive and flavoring agent. In the present study, using Ion Proton next generation sequencing technology we performed the small RNA sequencing, conserved and novel miRNA predictions of a wild and five cultivar genotypes of cardamom. Small RNA sequencing generated a total of 5,451,328 and 2,756,250 raw reads for wild and cultivar cardamom respectively. The raw data was submitted to SRA database of NCBI under the accession numbers and SRX2273863 (wild) and SRX2273862 (cultivars). The raw reads were quality filtered and predicted conserved and novel miRNAs for wild and cultivar cardamom. The predicted miRNAs, miRNA-targets and functional annotations might provide valuable insights into differences between wild progenitor and cultivated cardamom.
Highlights
Elettaria cardamomum (L.) Maton, or small cardamom referred as ‘queen of spices’, is a perennial herbaceous rhizomatous monocot of the family Zingiberaceae
Raw data in FASTQ file Small RNA sequencing of tissues of wild and cultivar cardamom Freshly collected leaf, stem, flower, flower buds and young fruits were used for RNA isolation, tissues were pooled, Small RNA seq libraries representing two genotypes of cardamom were sequenced and predicted known and novel miRNAs JNTBGRI, cardamom germplasm conservatory
The data presented in the article provides information on genome wide conserved and novel miRNA profiling and differential expression analysis of wild and cultivar genotypes of cardamom
Summary
Raw data in FASTQ file Small RNA sequencing of tissues of wild and cultivar cardamom Freshly collected leaf, stem, flower, flower buds and young fruits were used for RNA isolation, tissues were pooled, Small RNA seq libraries representing two genotypes of cardamom were sequenced and predicted known and novel miRNAs JNTBGRI, cardamom germplasm conservatory. The data presented in the article provides information on genome wide conserved and novel miRNA profiling and differential expression analysis of wild and cultivar genotypes of cardamom. This data identifies miRNAs participating in the negative regulation of significant transcription factors, enzymes and other mRNAs involved in plant development, stress responses and pathogenic resistance. The data shared in this article provides information on conserved and novel miRNAs and differential miRNA expression analysis between wild and cultivar genotypes of cardamom
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