Abstract
This article contains data on the variation in several physiological parameters of red blood cells (RBCs) donated by eligible glucose-6-phosphate dehydrogenase (G6PD) deficient donors during storage in standard blood bank conditions compared to control, G6PD sufficient (G6PD+) cells. Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured in RBCs throughout the storage period, with or without stimulation by oxidants, supplementation of N-acetylcysteine and energy depletion, following incubation of stored cells for 24h at 37°C. Apart from cell characteristics, the total or uric acid-dependent antioxidant capacity of the supernatant in addition to extracellular potassium concentration was determined in RBC units. Finally, procoagulant activity and protein carbonylation levels were measured in the microparticles population. Further information can be found in “Glucose 6-phosphate dehydrogenase deficient subjects may be better “storers” than donors of red blood cells” [1].
Highlights
Data on how several physiological parameters of stored red blood cells are similar in glucose 6-phosphate dehydrogenase deficient and sufficient donors
Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured in red blood cells (RBCs) throughout the storage period, with or without stimulation by oxidants, supplementation of Nacetylcysteine and energy depletion, following incubation of stored cells for 24 h at 37 °C
Intracellular reactive oxygen species (ROS) accumulation was similar in energy depleted (24 h/37 °C) G6PDÀ and glucose-6phosphate dehydrogenase (G6PD) þ stored RBCs while stimulation by tert-Butyl hydroperoxide and diamide oxidants resulted in statistically significant increase in ROS accumulation in the G6PDÀ group (n 1⁄46) compared to the G6PD þ group (n1⁄4 3) (Fig. 1)
Summary
Biology Biology of erythrocytes stored in blood banks for transfusion purposes Graphs, figures Cell fragility tests, hemolysis and total antioxidant capacity were measured spectrophotometrically. Physiological characteristics of stored RBCs and supernatants and malate variation were examined in RBC units donated by G6PDÀ and G6PD þ eligible donors. Intracellular reactive oxygen species (ROS) accumulation was similar in energy depleted (24 h/37 °C) G6PDÀ and G6PD þ stored RBCs while stimulation by tert-Butyl hydroperoxide (tBHP) and diamide oxidants resulted in statistically significant increase in ROS accumulation in the G6PDÀ group (n 1⁄46) compared to the G6PD þ group (n1⁄4 3) (Fig. 1). RBC fragility (both mean corpuscular fragility, MCF and mechanical fragility index, MFI) (Fig. 2) and the characteristics of the microparticles (accumulation, pro-coagulant activity and protein carbonylation index, PCI) (Fig. 3) were equal between the groups under examination throughout the storage period, while only slight differences were observed in the antioxidant capacity of the supernatant (Fig. 4). N-acetylcysteine (NAC) supplementation (at the concentration used) induced similar changes in both stored RBCs and supernatant (Fig. 6)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
