Abstract
The compaction of chromatin fibers regulates the accessibility of embedded DNA, highly associated with transcriptional activities [1]. Single molecule force spectroscopy has revealed the great details of the structural changes of chromatin fibers in the presence of external exerted force [2–7]. However, most of the studies focus on a specific force regime [2,3,8,9]. The data here show force-extension (FE) traces of chromatin fibers as measured with magnetic tweezers, covering the force regime from 0 pN to 27 pN. Those traces provide information for further studies at varied force regimes.
Highlights
Data on force-dependent structural changes of chromatin fibers measured with magnetic tweezers
Experimental factors Chromatin fibers were reconstituted with histone octamers purified from chicken erythrocytes and DNA fragments containing 25 copies 601 nucleosome positioning elements
Chromatin fibers were reconstituted with purified chicken histone octamers and DNA fragments containing 601 sequences through salt dialysis described previously [11]
Summary
Data on force-dependent structural changes of chromatin fibers measured with magnetic tweezers Single molecule force spectroscopy has revealed the great details of the structural changes of chromatin fibers in the presence of external exerted force [2,3,4,5,6,7]. Most of the studies focus on a specific force regime [2,3,8,9].
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