Abstract
Here we present additional data on the expression of lipoxygenases -5 and -12 in the normal and acetaminophen-damaged liver, which are associated with our manuscript recently published in Chemico-Biological Interactions on lipid metabolism and eicosanoid signaling pathways involved in acetaminophen-induced liver damage in a mouse model (http://dx.doi.org/10.1016/j.cbi.2015.10.019 [1]). It has been demonstrated that the expression of lipoxygenase-5 and leukotriene formation are increased in the livers of rats with carbon tetrachloride (CCl4)-induced cirrhosis (http://dx.doi.org/10.1053/gast.2000.17831 [2]). In addition, the lipoxygenase-12 is known to be expressed in the resident macrophage population of the liver (http://dx.doi.org/10.1016/S0014-5793(99)00396-8 [3]).Mice were injected with acetaminophen, and at 48 h their livers were processed for immunohistochemistry with anti-mouse lipoxygenase-5 and -12 antibodies. At the same time point, the RNA was also extracted from the liver to assess the expression of lipoxygenase-5 and -12 genes via qPCR analysis. Our results show that lipoxygenase-5 expression, but not that of lipoxygenase-12, changes significantly in the acetominophen-damaged liver.
Highlights
RNA was extracted from the liver to assess the expression of lipoxygenase-5 and -12 genes via qPCR analysis
Our results show that lipoxygenase-5 expression, but not that of lipoxygenase-12, changes significantly in the acetominophen-damaged liver
Analyzed Mice were injected with a dose of 400 mg/kg acetaminophen; Immunohistochemistry qPCR Mice were injected with a dose of 400 mg/kg acetaminophen and liver samples were collected at 48 h post-administration
Summary
Analyzed Mice were injected with a dose of 400 mg/kg acetaminophen; Immunohistochemistry qPCR Mice were injected with a dose of 400 mg/kg acetaminophen and liver samples were collected at 48 h post-administration. The data show that immunohistochemical and gene expression of lipoxygenase-5 and -12 are enhanced in the liver tissue during acetaminophen-induced damage. These data show that both lipoxygenase-5 and -12 signaling pathways are activated during acetaminophen-induced liver damage. The lipoxygenase-5 appeared to be weakly expressed in the normal liver parenchyma (Fig. 1A, 40 Â magnification), but at 48 h acetaminophen administration consistently enhanced its expression in the damaged livers[2], primarily around the centrilobular veins (Fig. 1B, 40 Â magnification). The lipoxygenase-12 appeared to stain weakly the normal liver parenchyma (Fig. 1C, 40 Â magnification), whereas acetaminophen administration enhanced, but only modestly, its expression at 48 h (Fig. 1D, 40 Â magnification), especially around the centrilobular veins[3]. When the expression of lipoxygenase-5 and -12 was examined in the control and acetaminophentreated livers, we found that at 48 h, the expression of both lipoxygenases was up-regulated, but significant differences were found only for lipoxygenase-5 (Fig. 1E and F)
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