Data on differentially expressed proteins in rock inhibitor-treated human trabecular meshwork cells using SWATH-based proteomics.

Chi-Ho To,Thomas Chuen Lam,Sze-Wan Shan,Hoi-Lam Li,Chi-Wai Do,W Daniel Stamer

doi:10.1016/j.dib.2020.105846

Abstract

Rho-associated coiled coil-forming protein kinase (ROCK) inhibitors represent a novel class of anti-glaucoma drugs because of their ocular hypotensive effects. However, the underlying mechanisms responsible for lowering intraocular pressure (IOP) are not completely clear. The protein profile changes in primary human trabecular meshwork (TM) cells after two days treatment with a ROCK inhibitor were studied using label-free SWATH acquisition. These results provided significant data of key protein candidates underlying the effect of ROCK inhibitor. Using the sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive TM proteome library. All raw data generated from IDA and SWATH acquisitions were uploaded and published in the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01254).

Highlights

Rho-associated coiled coil-forming protein kinase (ROCK) inhibitors represent a novel class of anti-glaucoma drugs because of their ocular hypotensive effects
The protein profile changes in primary human trabecular meshwork (TM) cells after two days treatment with a ROCK inhibitor were studied using label-free SWATH acquisition. These results provided significant data of key protein candidates underlying the effect of ROCK inhibitor
Ophthalmology Cell biology in eyes Fig. Table Information-dependent acquisition and data-independent acquisition using Quadrupole Time-of-Flight TripleTOF R 6600 mass spectrometer (SCIEX) Raw and analysed Proteins were isolated from primary human trabecular meshwork cells treated with of ROCK inhibitor for two days and untreated controls

Summary

Data accessibility

Ophthalmology Cell biology in eyes Fig. Table Information-dependent acquisition and data-independent acquisition using Quadrupole Time-of-Flight TripleTOF R 6600 mass spectrometer (SCIEX) Raw and analysed Proteins were isolated from primary human trabecular meshwork cells treated with of ROCK inhibitor for two days and untreated controls. ROCK inhibitor is a newly developed therapeutic agent for glaucoma, but the underlying mechanism for its ocular hypotensive action is not yet fully understood This project describes the use of SWATH MS to examine the underlying biological protein changes following ROCK inhibitor treatment of primary human trabecular meshwork (TM) cells. This knowledge may allow more specific anti-glaucoma drugs to be developed. Generation of the first published human TM cells ion library for future SWATH-MS study on eye diseases

Data description

Cell culture

Findings

Bioinformatics data analysis