Abstract
Ceramide pathway is a key regulator in cell proliferation and apoptosis and oxidative stress up-regulate ceramides. Acid ceramidase (ACDase), neutral sphingomyelinase (NSMase) and glucosylceramide synthase (GCS) are critical enzymatic systems in ceramide metabolism. Our data represent the comparative assessment of Hesperetin (Hst) and hesperetin-7-O-acetate (HTA) effects on A-494 renal carcinoma cells include cell survival, caspase-3 and 9 activities, total cellular ceramide and the activities of ACDase, NSMase, GCS and superoxide dismutase (SOD). Data reveals potentiating effects of both HTA and Hst on ceramide pathway and may offer a novel tool in human renal cell carcinoma therapy.
Highlights
Apoptosis, ceramide metabolism and oxidative stress in A-494 renal cell carcinoma cell line treated with hesperetin and hesperetin-7-O-acetate
Ceramide pathway is a key regulator in cell proliferation and apoptosis and oxidative stress up-regulate ceramides
Our data represent the comparative assessment of Hesperetin (Hst) and hesperetin-7-O-acetate (HTA) effects on A-494 renal carcinoma cells include cell survival, caspase-3 and 9 activities, total cellular ceramide and the activities of Acid ceramidase (ACDase), neutral sphingomyelinase (NSMase), glucosylceramide synthase (GCS) and superoxide dismutase (SOD)
Summary
Biology Apoptosis, ceramide, oxidative stress Graphs Fluorescence and spectrophotometry analysis Analyzed A-494 cells were treated with hesperetin or hesperetin-7-O-acetate Cells were exposed to different concentrations of hesperetin or hesperetin-7-O-acetate and after 48 h, they were prepared for spectrofluorometric assessment of related substance. Caspase and 9 activities, total cellular ceramide and the activities of ACDase, NSMase,GCS, and superoxide dismutase (SOD) determined with fluorescence and spectrophotometry analysis in examined cells which treated with different concentrations of Hst and HTA (0–200 μM). Both Hst and HTA reduced cell survival and in this regard, HTA was more potent than Hst. Caspase 3 activity with HTA and caspase 9 with Hst increased significantly in a dose-dependent fashion (Fig. 1). Caspase 9 is involved in intrinsic and caspase 3 in the common pathway of apoptosis [4] According to these data, total cellular ceramide increased somewhat at higher concentrations of Hst and HTA.
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