Data on calcium increases depending on stretch in dystrophic cardiomyocytes

E Aguettaz,J.J Lopez,A Krzesiak,B Constantin,C Cognard,S Sebille

doi:10.1016/j.dib.2016.08.011

E Aguettaz, J.J Lopez + Show 4 more

Open Access

https://doi.org/10.1016/j.dib.2016.08.011

Copy DOI

Abstract

In this data article, intracellular Ca2+ concentration ([Ca2+]i) was measured in isolated ventricular Wild Type (WT) and mdx cardiomyocytes in two different conditions: at rest and during the application of an axial stretch. Using a carbon microfibers technique, axial stretch was applied to mimic effects of physiological conditions of ventricular filling. A study of cation entry with the same experimental model and the manganese quenching method reported (i) a constitutive cation entry in mdx cardiomyocytes and (ii) the involvement of TRPV2 channels in axial-stretch dependant cation entry, “Axial stretch-dependent cation entry in dystrophic cardiomyopathy: involvement of several TRPs channels” (Aguettaz et al., 2016) [1].Here, the Ca2+ dye fluo-8 was used for [Ca2+]i measurement, in both resting and stretching conditions, using a perfusion protocol starting initially with a calcium free Tyrode solution followed by the perfusion of 1.8mM Ca2+ Tyrode solution. The variation of [Ca2+]i was found higher in mdx cardiomyocytes.

Highlights

In this data article, intracellular Ca2 þ concentration ([Ca2 þ]i) was measured in isolated ventricular Wild Type (WT) and mdx cardiomyocytes in two different conditions: at rest and during the application of an axial stretch
The Ca2 þ dye fluo-8 was used for [Ca2 þ]i measurement, in both resting and stretching conditions, using a perfusion protocol starting initially with a calcium free Tyrode solution followed by the perfusion of 1.8 mM Ca2 þ Tyrode solution
Biology Calcium regulation in cardiomyopathy Figures Confocal microscopy, BIORAD 1024 Raw, analysed Isolated mouse cardiomyocytes were axial stretched Intracellular calcium changes were recorded Poitiers, France Data is within this article

Summary

Data on calcium increases depending on stretch in dystrophic cardiomyocytes

Article history: Received 23 May 2016 Received in revised form 2 August 2016 Accepted 4 August 2016 Available online 10 August 2016. In this data article, intracellular Ca2 þ concentration ([Ca2 þ]i) was measured in isolated ventricular Wild Type (WT) and mdx cardiomyocytes in two different conditions: at rest and during the application of an axial stretch.

Value of the data

Findings

WT mdx