Abstract
Low bioavailability and/or survival at the injury site of transplanted stem cells necessitate its delivery using a biocompatible, biodegradable cell delivery vehicle. In this dataset, we report the application of a porous biocompatible, biodegradable polymer network that successfully delivers bone marrow stem cells (BMSCs) at the wound site of a murine excisional splint wound model. In this data article, we are providing the additional data of the reference article “Porous polymer scaffold for on-site delivery of stem cells – protects from oxidative stress and potentiates wound tissue repair” (Ramasatyaveni et al., 2016) [1]. This data consists of the characterization of bone marrow stem cells (BMSCs) showing the pluripotency and stem cell-specific surface markers. Image analysis of the cellular penetration into PEG–PU polymer network and the mechanism via enzymatic activation of MMP-2 and MMP-13 are reported. In addition, we provide a comparison of various routes of transplantation-mediated BMSCs engraftment in the murine model using bone marrow transplantation chimeras. Furthermore, we included in this dataset the engraftment of BMSCs expressing Sca-1+Lin−CD133+CD90.2+ in post-surgery day 10.
Highlights
bone marrow stem cells (BMSCs) isolated from murine bone marrow were characterized (Fig. 1A and B)
C57BL/J6 mice were used for the isolation of BMSC
MDA-MB-231 cells and BMSCs were cultured in 24-well plates with a density of 1 Â 105 cells/well
Summary
Graph, figures and images DSC Q200 and TG Q50 TA Instruments, flow-cytometer Beckman Coulter MoFlo Legacy, microscope – confocal: Olympus FV1000; fluoroscence: Olympus U-RFL-T, thermal cycler Takara TP600, applied biosystems 7900HT real time PCR Raw, analyzed Excisional splinting wound model, cell transplantation chimeras. Bone marrow stem cell (BMSCs) characterization, Scaffold-penetration by BMSCs-microscopic and zymographic analysis, BMSC transplantation chimeras (male cells into female mice)-qPCR analysis, BMSC engraftment at wound site and healing CSIR-Indian Institute of Chemical Technology, Hyderabad, India. This data will be helpful for the research community that evaluates various biomaterials as cell delivery vehicles for tissue engineering and regenerative medicines. We designed and synthesized a stable, biocompatible, pH-sensitive and enzymatically biodegradable castor oil-based porous polyethyleneglycol–polyurethane (PEG–PU) networks as scaffolds for delivery of BMSCs at an injury site that accelerates the wound tissue repair process ( $ 50% faster) [1] In this data, BMSCs isolated from murine bone marrow were characterized (Fig. 1A and B). Immunostaining analyses of wound tissue sections to evaluate engraftment of transplanted BMSCs are included (Fig. 7)
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