Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells

Junji Itou,Yoshiaki Matsumoto,Sunao Tanaka,Fumiaki Sato,Wenzhao Li,Masakazu Toi

doi:10.1016/j.dib.2015.09.037

Abstract

Three-dimensional (3D) cell culture is a powerful tool to study cell growth under 3D condition. To perform a simple test for anti-cancer drugs in 3D culture, visualization of non-proliferated cells is required. We propose a fluorescent imaging-based assay to analyze cancer cell proliferation in 3D culture. We used a pulse-labeling technique with a photoconvertible fluorescent protein Kaede to identify non-proliferated cells. This assay allows us to observe change in cell proliferation in 3D culture by simple imaging. Using this assay, we obtained the data of the effects of anti-cancer drugs, 5-fluorouracil and PD0332991 in a breast cancer cell line, MCF-7.

Highlights

Value of the dataPulse-labeling with fluorescent protein is useful technique to analyze cell proliferation in threedimensional culture
We propose a fluorescent imaging-based assay to analyze cancer cell proliferation in 3D culture
We obtained the data of the effects of anti-cancer drugs, 5-fluorouracil and PD0332991 in a breast cancer cell line, MCF-7

Summary

Value of the data

Pulse-labeling with fluorescent protein is useful technique to analyze cell proliferation in threedimensional culture. Non-proliferated cells are identified by pulse-labeling in three-dimensional culture. Our fluorescent imaging-based analysis can evaluate anti-cancer drug effects on cell proliferation. In 3D culture, change in cancer growth is analyzed by observation of the size and the morphology of a colony, and it is difficult to judge whether alterations are due to changes in cell proliferation and/or survival. To overcome this problem, simple proliferation assay is needed. We demonstrate a fluorescent imaging-based assay to analyze anti-cancer drug effects on 3D growth. Cells were treated with anti-cancer drugs, and subsequent changes in cell proliferation were analyzed

Material and methods

Pulse-labeling with Kaede-red

Findings

Treatment with 5-fluorouracil and PD0332991