Data in support of transcriptional regulation and function of Fas-antisense long noncoding RNA during human erythropoiesis.

Olga Villamizar,Janice M Riberdy,Donald S Torry,Derek A Persons,Reese Hofstrand,Yin-Yuan Mo,Andrew Wilber,Christopher B Chambers

doi:10.1016/j.dib.2016.03.106

Abstract

This paper describes data related to a research article titled, “Fas-antisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fas-mediated cell death” [1]. Long noncoding RNAs (lncRNAs) are increasingly appreciated for their capacity to regulate many steps of gene expression. While recent studies suggest that many lncRNAs are functional, the scope of their actions throughout human biology is largely undefined including human red blood cell development (erythropoiesis). Here we include expression data for 82 lncRNAs during early, intermediate and late stages of human erythropoiesis using a commercial qPCR Array. From these data, we identified lncRNA Fas-antisense 1 (Fas-AS1 or Saf) described in the research article. Also included are 5′ untranslated sequences (UTR) for lncRNA Saf with transcription factor target sequences identified. Quantitative RT-PCR data demonstrate relative levels of critical erythroid transcription factors, GATA-1 and KLF1, in K562 human erythroleukemia cells and maturing erythroblasts derived from human CD34+ cells. End point and quantitative RT-PCR data for cDNA prepared using random hexamers versus oligo(dT)18 revealed that lncRNA Saf is not effectively polyadenylated. Finally, we include flow cytometry histograms demonstrating Fas levels on maturing erythroblasts derived from human CD34+ cells transduced using mock conditions or with lentivirus particles encoding for Saf.

Highlights

Data in support of transcriptional regulation and function of Fas-antisense long noncoding RNA during human erythropoiesis
This paper describes data related to a research article titled, “Fasantisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fasmediated cell death” [1]
We include flow cytometry histograms demonstrating Fas levels on maturing erythroblasts derived from human CD34 þ cells transduced using mock conditions or with lentivirus particles encoding for Saf

Summary

Data accessibility

Methods to transduce human CD34 þ cells with lentiviral vectors encoding for expression of a functional lncRNA and subsequently monitor Fas receptor levels by flow cytometry. We provide expression data for a focused set of lncRNAs during culture-induced differentiation of human CD34 þ cells into erythroblasts (Table 1). We include relative transcript levels of GATA-1 and KLF1 in K562 human erythroleukemia cells and maturing human erythroblasts We share end point and quantitative RT-PCR analysis of cDNA prepared from total RNA isolated from human erythroblasts using random hexamers versus oligo(dT) (Fig. 4). We include flow cytometry histograms demonstrating Fas surface levels on maturing erythroblasts derived from human CD34 þ cells transduced using mock conditions or Saf-encoding lentivirus particles (Fig. 5). In bold are lncRNAs with a 2-fold *decrease or **increase on both days 6 and 10; ND, not detected

LncRNA quantitative RT-PCR array

Relative expression of GATA-1 and KLF1

Saf polyadenylation status