Abstract
This data article contains data related to the research article entitled, "Protocol for rat single muscle-fiber isolation and culture" by Komiya et al. [1]. It has yet to be shown whether adult myosin heavy chain (MyHC) isoforms are expressed at a readily detectable level in cultured myotubes. In this study, we examined whether adult MyHC isoforms could be detected in myotubes differentiated from rat satellite cells using a Western blotting assay and specific antibodies against slow MyHC, fast MyHC and pan-MyHC. Results showed that slow adult MyHC isoforms were faintly detected in myotubes, suggesting that rat myotubes express adult MyHC isoforms although that amount is very low.
Highlights
Data accessibilityThe low but detectable amount of adult slow myosin heavy chain (MyHC) was confirmed in cultured myotubes differentiated from rat satellite cells for 6 days by Western blotting
This data article contains data related to the research article entitled, "Protocol for rat single muscle-fiber isolation and culture" by Komiya et al [1]
We found that immature neonatal and embryonic myosin heavy chain (MyHC) isoforms were dominant in myotubes differentiated from rat satellite cells [1]
Summary
The low but detectable amount of adult slow MyHC was confirmed in cultured myotubes differentiated from rat satellite cells for 6 days by Western blotting. The regulation of adult slow MyHC isoforms expression could be evaluated by Western blotting even though immature cultured myotubes. Undetectable level of adult fast MyHC isoforms appeared to be contained in myotubes in this experimental condition
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