Abstract
The final differentiation of the male germ cell occurs in the epididymal duct where the spermatozoa develop the ability to be motile and fertilize an ovum. Understanding of these biological processes is the key to understanding and controlling male fertility. Comparative studies between several epididymal maturation states could be an informative approach to finding sperm modifications related to maturation and fertility. Here we show the data from differential peptidomic/proteomic analyses on spermatozoa isolated from 4 epididymal regions (immature to mature stage) using a profiling approach based on MALDI-TOF mass spectrometry and, combined to top-down MS in order to identify peptidoforms and proteoforms. By this way, 172m/z peaks ranging between 2 and 20 kDa were found to be modified during maturation of sperm. A total of 62m/z were identified corresponding to 32 different molecular species. The interpretation of these data can be found in the research article published by Labas and colleagues in the Journal of Proteomics in 2014 [1].
Highlights
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We show the data from differential peptidomic/proteomic analyses on spermatozoa isolated from 4 epididymal regions using a profiling approach based on MALDI-TOF mass spectrometry and, combined to top-down MS in order to identify peptidoforms and proteoforms
Peptidomic analysis of boar epididymal spermatozoa and molecular phenotypes at different maturation stages Top down raw data and.csv tables with identified proteins Experiments performed on a LTQ Orbitrap Velos Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) Raw data .csv results tables of top-down identification Boar spermatozoa from 4 different regions of the epididymis Epididymal spermatozoa peptidome (Sus scrofa) and investigation of the boar sperm maturation process N/A Data are available here and via the PRIDE partner repository with the dataset identifier PXD001303
Summary
The epididymides were collected from four one-year-old adult boars. The luminal contents of the tubules of four epididymal regions (E2, E4, E6 and E9) were collected by micro perfusion [2]. The pellets were washed once with PBS (140 mM NaCl, 15 mM KCl, 7 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.4), centrifuged and resuspended in PBS. The spermatozoa were centrifuged on 40% Percoll in PBS. The pellets were washed again with PBS and resuspended in 20 mM Tris–HCl, pH 6.8, and 260 mM sucrose (Tris– sucrose buffer (TS)) (Fig. 1)
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