Abstract
2D-DIGE analysis coupled with mass spectrometry is a global, without a priori, comparative proteomic approach particularly suited to identify and quantify enzymes isoforms and structural proteins, thus making it an efficient tool for the characterization of the changes in cell phenotypes that occur in physiological and pathological conditions. In this data article in support of the research article entitled “Metabolic reprogramming in transformed mouse cortical astrocytes: a proteomic study” [1] we illustrate the changes in protein profile that occur during the metabolic reprogramming undergone by cultured mouse astrocytes in a model of in-vitro cancerous transformation [2].
Highlights
2D-DIGE analysis coupled with mass spectrometry is a global, without a priori, comparative proteomic approach suited to identify and quantify enzymes isoforms and structural proteins, making it an efficient tool for the characterization of the changes in cell phenotypes that occur in physiological and pathological conditions
Our results suggest that transformation causes major losses of astrocyte-specific proteins and functions and the acquisition of metabolic adaptations that favor intermediate metabolites production for increased macromolecule biosynthesis
Cultures of mouse normal astrocytes were prepared from cortices of 1-to-2-day-old C57Bl6/J mice according to protocols described by Sharif in 2007 [3] and Prevot in 2005 [4] with slight adaptations
Summary
Cultures of mouse normal astrocytes were prepared from cortices of 1-to-2-day-old C57Bl6/J mice according to protocols described by Sharif in 2007 [3] and Prevot in 2005 [4] with slight adaptations. Cultures were established in MEM medium (Gibco, Life-technologies, UK) containing 10% fetal calf serum (FCS) (Lonza, BioWhittakers, Belgium). When confluence was reached again, the cells were maintained in serum-free MEM medium for 48 h, rinsed twice in PBS, and either extracted for protein sample preparation or frozen until further use. Ethanesulfonic acid (HEPES) buffer, 5 IU/ml penicillin and 5 mg/ml streptomycin, and the B27 complement (Gibco, ref 12587-010). This medium was renewed once a week. Due to the selection process and growth conditions, all the cells generated from the irradiated cultures were transformed astrocytes
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