Abstract
Sport is a type of comprehensive activity that the human body consciously engages in to improve physical fitness. Proteomics is a comprehensive technology dedicated to the study of all protein profiles expressed by a species, individual organ, tissue, or cell under specific conditions and specific times. Proteomics is a science that studies the protein composition of cells, tissues, or organisms and their changing laws with proteomics as the research object. Related technologies are now widely used in sports and other fields. The purpose of this article is to study myocardial proteomic technology and its application in sports. During the research process, the main methods used in this study are literature survey and controlled experiment. The results achieved and the problems in this field, followed by selecting 30 SD rats into 3 groups for control experiments. The results of the study showed that among the three groups of rats, the left ventricular ejection fraction of the sham operation group was the highest, which was 7.7% and 4.6% higher than that of the operation group and the model group, respectively. The operation group had the highest left ventricular short axis shortening rate, and the left ventricle diastolic inner diameter is the longest. It can be seen that myocardial proteomics can accurately reflect the heart condition of rats. In addition, the length, diastolic velocity, and diastolic time of cardiomyocytes of the three groups of rats were different. Among them, the cardiomyocytes of the operation group had the longest time and the longest diastolic time, which were 37.1% and 8.5% higher than those of the sham operation group and the model group.
Highlights
Sports are a comprehensive activity carried out by the human body consciously for improving physical fitness
Sports will cause a series of changes in the internal environment of the human body, which in turn affects the expression of related genes in other tissues and the synthesis of related proteins before and after exercise
If the sample is found to have good repeatability and stable preparation, it can be used for the analysis of different protein spots
Summary
Sports are a comprehensive activity carried out by the human body consciously for improving physical fitness. Sports will cause a series of changes in the internal environment of the human body, which in turn affects the expression of related genes in other tissues and the synthesis of related proteins before and after exercise. Proteomic techniques and methods can be applied to explore the differences in the proteome of different organs and tissues in the human body under physical exercise conditions and have become an important way to study the human body’s response to exercise and the molecular biological mechanisms of exercise. Hsp in the myocardium increased significantly after endurance exercise, and the phosphorylation of Hsp serine 16 (HSP20-PS16) increased These studies have shown that endurance training during exercise helps to improve the expression and phosphorylation of Hsp in cardiomyocytes. The mechanism of the protective effect of the heat shock protein expression on cardiomyocytes during exercise is still unclear, and further experimental studies are needed
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