Data from <i>Pten</i>-Null Tumors Cohabiting the Same Lung Display Differential AKT Activation and Sensitivity to Dietary Restriction

Abstract

<div>Abstract<p><i>PTEN</i> loss is considered a biomarker for activated phosphoinositide 3-kinase (PI3K)/AKT, a pathway frequently mutated in cancer, and was recently shown to confer resistance to dietary restriction. Here, we show that <i>Pten</i> loss is not sufficient to drive AKT activation and resistance to dietary restriction in tumors with low growth factor receptor levels. We describe a murine <i>Pten</i>-null <i>Kras</i>-driven lung cancer model that harbors both dietary restriction–resistant, higher-grade, bronchiolar tumors with high AKT activity, and dietary restriction–sensitive, lower-grade, alveolar tumors with low AKT activity. We find that this phenotype is cell autonomous and that normal bronchiolar cells express higher levels of insulin-like growth factor-I receptor (IGF-IR) and of ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), an endoplasmic reticulum enzyme known to modulate growth factor receptor levels. Suppression of <i>ENTPD5</i> is sufficient to decrease IGF-IR levels and sensitize bronchiolar tumor cells to serum <i>in vitro</i> and to dietary restriction <i>in vivo</i>. Furthermore, we find that a significant percentage of human non–small cell lung carcinomas (NSCLC) have low AKT activity despite <i>PTEN</i> loss.</p><p><b>Significance:</b> Our studies point to a heterogeneity of AKT activation in the same murine <i>Pten</i>-null lung tissue and in human NSCLC, further underscoring the challenges of personalizing cancer therapy based solely on cancer genotype. Our findings therefore indicate that the tumor response to anticancer therapies, including dietary restriction, needs to be based on PI3K/AKT activity <i>per se</i>, rather than on genetic alterations in the PTEN/PI3K pathway. <i>Cancer Discov; 3(8); 908–21. ©2013 AACR</i>.</p><p>This article is highlighted in the In This Issue feature, p. 826</p></div>