Abstract
<div>Abstract<p>New Western-style diet 1 (NWD1), a purified diet establishing mouse exposure to key nutrients recapitulating levels that increase human risk for intestinal cancer, reproducibly causes mouse sporadic intestinal and colonic tumors reflecting human etiology, incidence, frequency, and lag with developmental age. Complex NWD1 stem cell and lineage reprogramming was deconvolved by bulk and single-cell RNA sequencing, single-cell Assay for Transposase-Accessible Chromatin using sequencing, functional genomics, and imaging. NWD1 extensively, rapidly, and reversibly, reprogrammed Lgr5<sup>hi</sup> stem cells, epigenetically downregulating <i>Ppargc1a</i> expression, altering mitochondrial structure and function. This suppressed Lgr5<sup>hi</sup> stem cell functions and developmental maturation of Lgr5<sup>hi</sup> cell progeny as cells progressed through progenitor cell compartments, recapitulated by <i>Ppargc1a</i> genetic inactivation in Lgr5<sup>hi</sup> cells <i>in vivo</i>. Mobilized <i>Bmi1+, Ascl2<sup>hi</sup></i> cells adapted lineages to the nutritional environment and elevated antigen processing and presentation pathways, especially in mature enterocytes, causing chronic, protumorigenic low-level inflammation. There were multiple parallels between NWD1 remodeling of stem cells and lineages with pathogenic mechanisms in human inflammatory bowel disease, also protumorigenic. Moreover, the shift to alternate stem cells reflects that the balance between Lgr5-positive and -negative stem cells in supporting human colon tumors is determined by environmental influences. Stem cell and lineage plasticity in response to nutrients supports historic concepts of homeostasis as a continual adaptation to environment, with the human mucosa likely in constant flux in response to changing nutrient exposures.</p>Implications:<p>Although oncogenic mutations provide a competitive advantage to intestinal epithelial cells in clonal expansion, the competition is on a playing field dynamically sculpted by the nutritional environment, influencing which cells dominate in mucosal maintenance and tumorigenesis.</p></div>
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