Data for the gene expression profiling and alternative splicing events during the chondrogenic differentiation of human cartilage endplate-derived stem cells under hypoxia.

Yuan Yao,Yue Zhou,Jin Shang,Weilin Song,Qiyue Deng,Huan Liu

doi:10.1016/j.dib.2016.04.037

Yuan Yao, Yue Zhou + Show 4 more

Open Access

https://doi.org/10.1016/j.dib.2016.04.037

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Abstract

This article contains relevant data of the research article titled Global profiling of the gene expression and alternative splicing events during the hypoxia-regulated chondrogenic differentiation in human cartilage endplate-derived stem cells (Yao et al., 2016) [1]. The data show global profiling of the DEGs (Differentially expressed genes) and AS (Alternative splicing) events during the hypoxia-regulated chondrogenesis of CESCs (human cartilage endplate-derived stem cells) by using Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) system. In addition, the enriched GO (Gene Ontology) functions and signaling pathways are listed. The information presented here includes the information of patients from which the clinical samples are obtained, the list of primers used for validation, the identification, GO and KEGG analysis of DEG and AS events.

Highlights

This article contains relevant data of the research article titled Global profiling of the gene expression and alternative splicing events during the hypoxia-regulated chondrogenic differentiation in human cartilage endplate-derived stem cells (Yao et al, 2016) [1]
AGCC (Affymetrix GeneChips Command Console) and EC (Affymetrix Expression Console) software were employed to analyze the data of microarray
The data are valuable for the genome-wide investigation of the mechanisms underlying the differentiation fate of cartilage endplate-derived stem cells (CESCs) in response to physiological hypoxia

Summary

Data accessibility

Analyzed Human cartilage endplate-derived stem cells (CESCs) were treated with osteogenic differentiation medium under normoxia and hypoxia respectively. The data is available with this article. The data are valuable for the genome-wide investigation of the mechanisms underlying the differentiation fate of CESCs in response to physiological hypoxia. 818 DEGs and 16259 AS events were identified during the CESCs osteogenesis under hypoxia compared with that under normoxia. The GO and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis of DEGs and AS events determined the significantly enriched GO terms (biological process, molecular function and cellular component) and signaling pathways, which is beneficial to the understanding of mechanisms underlying cartilage endplate degeneration. The data is available with this article and is related to [1]

Experimental design

CESCs isolation and culture

Induction and oxygen deprivation

Criteria for detecting DEGs and ASGs

Semi-quantitative RT-PCR