Abstract
This article presents data related to the research article titled, ‘Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering’ (Owen et al., in press) [1]. This data article contains excel files with the results obtained during the mechanical characterisation of 20 acrylate-based PolyHIPE compositions, giving the Young’s modulus, ultimate tensile stress and strain at failure for each specimen tested. Also included are the measurements taken to determine the degree of openness (DOO) of each composition, and the data for the cell viability and alkaline phosphatase (ALP) activity on the emulsion templated scaffolds.
Highlights
This article presents data related to the research article titled, ‘Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering’ (Owen et al, in press) [1]
Cell viability was determined using resazurin reduction assay and osteogenic differentiation by measuring alkaline phosphatase activity normalised to DNA content
Culture media was removed from the scaffolds, they were washed twice in PBS and 500 ml of cell digestion buffer (10 v/v % cell assay buffer (1.5 M Tris–HCl, 1 mM ZnCl2, 1 mM MgCl2 in deionised water, 1% TritonX100, in diH2O) was added to each scaffold and incubated for 30 min
Summary
Fluorescence in vitro assays (FLX800 plate reader, BIO-TEK). Absorbance in vitro assays (ELX800 plate reader, BIO-TEK) Analyzed Tensile specimens fabricated from photocured sheets via laser cutting. Three compositions with distinctly different stiffness were selected for scaffold manufacture and structured into scaffolds with a multiscale porosity using microstereolithography. Cell viability was determined using resazurin reduction assay and osteogenic differentiation by measuring alkaline phosphatase activity normalised to DNA content. The data described here may be helpful to researchers who are performing research where specific mechanical properties are required from a porous polymer, both within tissue engineering and other fields. Wet mechanical properties for the three compositions selected for cell culture Degree of openness values calculated from SEM images using the measurement tool in ImageJ for the physical characterization. Resazurin reduction cell viability assay fluorescence values Normalised ALP activity values
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